Corvinus
Corvinus

Detection of self-complementary inverted repeats by single forward primer driven PCR

Kupi, Tünde and Deák, Tamás and Bisztray, György Dénes and Szegedi, Ernő (2014) Detection of self-complementary inverted repeats by single forward primer driven PCR. Acta Biologica Szegediensis, 58 (1). pp. 65-68.

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Abstract

Inverted repeat gene structures designed for silencing functional genes have been widely used both in academic and applied research. The correct orientations of such structures are usually validated with restriction analysis and/or sequencing. We speculated that the inverted repeat nature of such constructs can be shown by a simple PCR reaction with a single forward primer. To test this hypothesis five different constructs were established from grapevine sequences in a hairpin-intron style silencing system. We were able to amplify the appropriate products in each case. Thus a forward-primed PCR alone may be sufficient to prove the inverted repeat nature of the desired constructs.

Item Type:Article
Uncontrolled Keywords:agrobacterium tumefaciens, crown gall, Phire Taq polymerase, plant diseases, RNA interference
Subjects:Viticulture
Plant protection
Projects:OTKA K83121
ID Code:2192
Deposited By: MTMT SWORD
Deposited On:14 Jan 2016 08:16
Last Modified:14 Jan 2016 08:19

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