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Corvinus

Silencing Agrobacterium oncogenes in transgenic grapevine results in strain-specific crown gall resistance

Galambos, Anikó and Zok, Anikó and Kuczmog, Anett and Oláh, Róbert and Putnoky, Péter and Szegedi, Ernő (2013) Silencing Agrobacterium oncogenes in transgenic grapevine results in strain-specific crown gall resistance. Plant Cell Reports, 32 (11). pp. 1751-1757. DOI 10.1007/s00299-013-1488-0

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Abstract

Crown gall disease of grapevine induced by Agrobacterium vitis or Agrobacterium tumefaciens causes serious economic losses in viticulture. To establish crown gall-resistant lines, somatic proembryos of Vitis berlandieri × V. rupestris cv. 'Richter 110' rootstock were transformed with an oncogene-silencing transgene based on iaaM and ipt oncogene sequences from octopine-type, tumor-inducing (Ti) plasmid pTiA6. Twentyone transgenic lines were selected, and their transgenic nature was confirmed by polymerase chain reaction (PCR). These lines were inoculated with two A. tumefaciens and three A. vitis strains. Eight lines showed resistance to octopine-type A. tumefaciens A348. Resistance correlated with the expression of the silencing genes. However, oncogene silencing was mostly sequence specific because these lines did not abolish tumorigenesis by A. vitis strains or nopaline-type A. tumefaciens C58.

Item Type:Article
Subjects:Viticulture
DOI:10.1007/s00299-013-1488-0
ID Code:2194
Deposited By: MTMT SWORD
Deposited On:18 Jan 2016 08:23
Last Modified:18 Jan 2016 08:23

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